mRNA NANOCAPSULE

An mRNA nanocapsule, comprising:

• a plurality of capsid proteins, self-assembling into a virus-like particle, wherein each of the capsid proteins comprises an RNA-binding motif;
• one or more first RNA molecules, comprising an mRNA encoding a Cas13 protein and a capsid protein binding tag, wherein the capsid protein binding tag recognizes and binds to the RNA-binding motif of one of the capsid proteins;
• and
• one or more second RNA molecules, encoded by a nucleotide sequence comprising a targeting ta sequence reverse and complementary to a targeted site, a Cas13 protein recognition sequence, and the capsid protein binding tag recognizing and binding to the RNA-binding motif of one of the capsid proteins;
• wherein the capsid protein binding tag is encoded by a virus-like particle recognition sequence as denoted by SEQ ID NO:
• 1, and the RNA-binding motif comprises an amino acid sequence as denoted by SEQ ID NO:
• 15;
• and
• wherein the one or more first RNA molecules and the one or more second RNA molecules are encapsulated in the virus-like particle.