Systems and methods for exosome delivery of micrornas for cellular reprogramming
OwnerConrad P. Hodgkinson, Hualing Sun +1inventors
InventorsConrad P. Hodgkinson, Hualing Sun, Victor J. Dzau
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Abstract
The present disclosure describes, in part, exosomes comprising miRNA for the reprogramming of fibroblasts and methods of using the same.
Claims35
A loaded exosome comprising an endothelial exosome, and at least one exogenous miR within said endothelial exosome.
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An engineered cell for producing exosomes, comprising a mammalian endothelial cell and a cassette for expression of one or more exogenous miR.
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A system for cell delivery comprising a loaded exosome and an inhibitor of MDFIC expression.
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A method for manufacturing a loaded exosome comprising:
- providing an endothelial cell comprising an inhibitor of MDFIC expression and at least one exogenous miR;
- inducing the cell to produce loaded exosomes comprising the at least one exogenous miR;
- and harvesting the loaded exosome.
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Dependencies are inferred from the claim text.