US 2024/0191211 A1 · BIOLUMINESCENCE-TRIGGERED PHOTOCATALYTIC LABELING

Abstract

Provided herein are systems, methods, and compositions for bioluminescence-triggered catalysis of bioorthogonal labeling chemistries in a proximity dependent manner. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts or photosensitizers, activatable labels, and systems thereof, and methods of catalytically activating the activatable labels via bioluminescence-triggered catalysis.

Claims33

Dependent claims

A system comprising:

(a) (i) a bioluminescent protein having at least 70% sequence identity with SEQ ID NO:
1 or a circularly permuted variant thereof or (ii) structurally-complementary components of a bioluminescent complex wherein the structurally-complementary components collectively comprise at least 70% sequence identity with SEQ ID NO:
2;
(b) a coelenterazine luminophore or a derivative thereof, wherein the bioluminescent protein or complex catalyzes emission of a first wavelength of light from the luminophore upon interaction therewith;
(c) a photocatalyst, wherein the photocatalyst is activated by exposure to light of the first wavelength;
and
(d) an activatable label, wherein the activatable label is converted into an activated label when in proximity to the activated photocatalyst.

4 more elements

Dependencies are inferred from the claim text.

Bioluminescence-triggered photocatalytic labeling

Life of this patent

Bioluminescence-triggered photocatalytic labeling

Life of this patent

Abstract

Claims33