Separation media and purification methods for carbohydrate binding domain containing molecules
AssigneeDonaldson Company, Inc.
InventorsJinxiang Zhou, Graham Temples, Daniel Henn
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Abstract
Separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to a carbohydrate recognizing domain. Methods of making the separation media and methods of using the separation media are disclosed.
Claims20
-34. (canceled)
A separation media comprising:
- a support substrate;
- and
- a plurality of separation ligands for formula SL1 or SL2
- Rp1-z (SL1)
- Rp3-Sp-Rp4-Z (SL2)
- wherein:
- Z is a separation group comprising an affinity group, the affinity group comprising a carbohydrate, a small molecule, or both;
- and
- Rp1, Rp3, and Rp4 each independently comprise the reaction product of any one of RpA, RpB, RpC, RpD, RpE, RpF, RpG, RpH, RpI, RpJ, RpK, or an isomer thereof, wherein RpA, RpB, RpC, RpD, RpE, RpF, RpG, RpH, RpI, RpJ, RpK are represented by:
- wherein:
- U0, U1, U2, U3, U4, US, U6, and U7 are each independently NH, O, or S;
- Sp is a spacer comprising a divalent organic group, and
- wherein the separation media is configured for isolating a target molecule comprising a carbohydrate binding domain.
The separation media of claim 35, wherein the carbohydrate comprises a glycan. 44 The separation media of claim 43, wherein the glycan has a linear structure.
The separation media of claim 44, wherein the glycan comprises a terminal sialic acid and an adjacent saccharide, and wherein the terminal sialic acid and the adjacent saccharide are covalently linked through a linkage.
Dependencies are inferred from the claim text.