Sonosensitization

A method for treating diffuse intrinsic pontine glioma in a human subject in need thereof, comprising selectively inducing a directly cytotoxic effect or apoptosis within diffuse intrinsic pontine glioma tissue in a subject, the method comprising:

• a) intravenously administering from 5 mg/kg to 750 mg/kg of gamma-irradiated 5-aminolevulinic acid (5-ALA), or a pharmaceutically acceptable salt thereof, to the subject;
• and
• b) exposing the tissue to ultrasound energy (“sonicating”) using a focused ultrasound (FUS) device, at a frequency of about 0.1 MHz to about 3 MHZ, at an intensity at the ultrasound beam focus of about 3 W/cm2 to about 100 W/cm2, wherein the tissue is sonicated at a plurality of individual points.