Substitution of nucleotide bases in self-amplifying messenger ribonucleic acids
AssigneeGlaxosmithkline Biologicals SA
InventorsGiulietta MARUGGI, Qiongman KONG, Varnika ROY, Dong YU, Meng ZHANG
Life of this patent
Estimating the term…
Abstract
Provided herein are RNA that: are collectively self-amplifying in an intracellular environment, comprise N1-methyl-pseudouridines and uridines, and have a mole percentage or mole proportion of the N1-methylpseudouridines to the total of the uridines and the Ni-methylpseudouridines or a mole ration of the N1-methylpseudouridines to the uridines.
Claims39
A self-amplifying messenger (SAM) ribonucleic acid (RNA) comprising N1-methylpseudouridines, uridines, a first RNA segment, and a second RNA segment;
- the first RNA segment comprising a heterologous nucleic acid;
- the second RNA segment encoding one or more proteins capable of replicating the SAM RNA in an intracellular environment;
- the SAM RNA having a first mole percentage of the N1-methylpseudouridines to the total of the N1-methylpseudouridines and the uridines from 15% to 75%.
A plurality of auto-amplifying messenger (AAM) RNAs comprising N1-methylpseudouridines, uridines, a first RNA, and one or more second RNA;
- the first RNA comprising a heterologous nucleic acid;
- the one or more second RNA encoding one or more proteins capable of replicating the AAM RNA in an intracellular environment;
- the plurality of AAM RNAs having a first mole percentage of the N1-methylpseudouridines to the total of the N1-methypseudouridines and uridines from 15% to 75%.
Dependencies are inferred from the claim text.