Graphene oxide nanoparticles and methods of use for stimulating immune responses
AssigneeGeorgia State University Research Foundation, Inc.
InventorsBaozhong Wang, Chunhong Dong
Life of this patent
Estimating the term…
Abstract
Disclosed are functionalized graphene oxide nanoparticles and methods their use in inhibiting and treating microbial infection. The disclosure herein further provides for methods of making functionalized graphene oxide nanoparticles and said nanoparticles further incorporating microbial antigens for inducing antigen specific immune responses.
Claims44
A composition comprising i) a functionalized graphene oxide (GO) nanoparticle comprising polyethyleneimine (PEI) (GO-PEI, GP) and ii) a microbial antigen, vaccine, and/or pharmaceutical agent.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method of making a vaccine to a microbial antigen comprising
- a. obtaining a graphene oxide (GO) powder;
- b. sonicating GO flakes in an ice bath until the GO particles are less than 500 nm in diameter using tip ultrasonication;
- c. adding polyethyleneimine (PEI) and sonicating;
- d. activating the GO-PEI with EDC;
- and
- e. adding the microbial antigen to the activated GO-PEI particles at a GO-PEI to Ag weight-to-weight ratio ranging from 10:1 to 1:10.
(canceled)
Dependencies are inferred from the claim text.