Recombinant viral expression vectors and methods of use
AssigneeNorth Carolina State University
InventorsAnna E. Whitfield, Surapathrudu Kanakala, Kathleen M. Martin
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Abstract
The present disclosure provides materials and methods relating to recombinant viral expression vectors. In particular, the present disclosure provides novel recombinant viral expression vectors comprising an RNA virus backbone that encodes a virus capable of infecting a target organism and expressing a polynucleotide-of-interest in that target organism. The novel viral vector constructs provided herein are a versatile expression tool for interrogating gene function and an efficient delivery platform for gene editing technology.
Claims27
A recombinant viral expression vector comprising:
- a negative strand RNA virus (NSV) backbone comprising polynucleotide sequences encoding core proteins;
- and
- at least one expression cassette comprising an exogenous polynucleotide sequence flanked by viral gene junctions from the NSV backbone;
- wherein the NSV backbone encodes a virus capable of infecting a target organism and expressing the exogenous polynucleotide sequence.
Dependencies are inferred from the claim text.