Compositions and methods for increased protein production in bacillus licheniformis
AssigneeDanisco US Inc.
InventorsMarc Anton Bernhard Kolkman, Steven D. Doig, Ryan L. Frisch, Hongxian He, Frank Wouter Koopman, Chris Leeflang
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Abstract
The present disclosure is generally related to compositions and methods for obtaining Bacillus licheniformis cells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modified Bacillus licheniformis cells/strains derived from parental B. licheniformis cells/strains comprising a variant rghR2 gene.
Claims6
1-6. (canceled)
A modified Bacillus licheniformis cell derived from a parental B. licheniformis cell, wherein the modified cell comprises a genetic modification which disrupts, deletes, inactivates, or down-regulates:
- (i) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO:
- 4,
- (ii) a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEQ ID NO:
- 16,
- (iii) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO:
- 4;
- and a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEQ ID NO:
- 16, or
- (iv) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO:
- 4, a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEQ ID NO:
- 16, a yvzC gene encoding a yvzC protein comprising at least 90% sequence identity to SEQ ID NO:
- 18 and/or a Bli03644 gene encoding a Bli03644 protein comprising at least 90% sequence identity to SEQ ID NO:
- 20,
- wherein the modified cell produces an increased amount of a protein of interest relative to the parental cell.
8-14. (canceled)
Dependencies are inferred from the claim text.