Method for producing cell sheet

Abstract

A method for preparing a cell sheet having a durable strength in practical use with good reproducibility within a period of time as short as possible, regardless of a culture medium additive or a type of culture vessel to be used is provided. The method for producing a cell sheet comprises a step of culturing mesenchymal stem cells in a medium containing an ascorbic acid species at 0.3 mM or higher and a ROCK (Rho-associated coiled-coil forming kinase) inhibitor to prepare mesenchymal stem cells in a sheet-like form in a culture vessel, wherein the ascorbic acid species is a water-soluble ascorbic acid derivative or a mixture of a water-soluble ascorbic acid derivative and ascorbic acid.

Dependencies are inferred from the claim text.