Guest-host supramolecular assembly of injectable hydrogel nanofibers for cell encapsulation
AssigneeUniversity of Virginia Patent Foundation
InventorsSteven R. Caliari, Beverly Miller
Life of this patent
Estimating the term…
Abstract
Injectable fibrous hydrogel are provided. The injectable fibrous hydrogels include a guest macromer of a hyaluronic acid (HA) backbone and host macromer of a HA backbone, the guest macromer is a HA electrospun hydrogel nanofiber functionalized with adamantane (Ad), and the host macromer is a HA electrospun hydrogel nanofiber functionalized with β-cyclodextrin (CD). Injectable formulations that include the fibrous hydrogels are also provided, as are methods of making and using the same.
Claims31
An injectable fibrous hydrogel, the injectable fibrous hydrogel comprising a guest macromer of a hyaluronic acid (HA) backbone and host macromer of a HA backbone, wherein the guest macromer comprises a HA electrospun hydrogel nanofiber functionalized with adamantane (Ad), wherein the host macromer comprises a HA electrospun hydrogel nanofiber functionalized with β-cyclodextrin (CD).
The injectable formulation of 13, wherein the injectable formulation is configured for injection into a tissue of a subject, optionally a fibrous tissue, optionally a muscle, tendon, or ligament tissue.
A method of forming a fibrous hydrogel nanofiber in a polymer solution, the method comprising:
- methacrylating a hyaluronic acid (HA) backbone via methacrylate esterification with a primary hydroxyl group of a sodium HA to form methacrylated HA (MeHA);
- synthesizing adamantane (Ad)-modified MeHA (Ad-MeHA) and 8-cyclodextrin (CD)-modified MeHA (CD-MeHA) by anhydrous coupling;
- electrospinning the fibrous hydrogel nanofiber in the polymer solution;
- and
- crosslinking the fibrous hydrogel nanofiber by exposure to ultraviolet (UV) light.
Dependencies are inferred from the claim text.