US 2024/0177803 A1 · COMPREHENSIVE IMMUNOPROFILING OF PERIPHERAL BLOOD

Abstract

Aspects of the disclosure relate to methods, systems, and computer-readable storage media, that are useful for characterizing subjects having cancer. The disclosure is based, in part, on methods for immunoprofiling a cancer subject and the subject's prognosis and/or likelihood of responding to an immunotherapy based upon analysis of leukocyte populations in the peripheral blood of the subject.

Claims30

Dependent claims

A method for determining a leukocyte immunoprofile type of a subject having, suspected of having, or at risk of having cancer, the method comprising:

using at least one computer hardware processor to perform:
obtaining cytometry data or RNA expression data from a biological sample obtained from the subject;
processing the cytometry data or the RNA expression data to determine cell composition percentages for at least 20 cell types listed in Table 4;
generating a leukocyte signature for the subject using the determined cell composition percentages for the at least 20 cell types, the leukocyte signature comprising the cell composition percentages for the at least 20 cell types;
and
identifying, using the leukocyte signature and from among a plurality of leukocyte immunoprofile types, a leukocyte immunoprofile type for the subject.

3 more elements

A method for determining a leukocyte immunoprofile type of a subject having, suspected of having, or at risk of having cancer, the method comprising:

using at least one computer hardware processor to perform:
obtaining flow cytometry data for white blood cells (WBC) isolated from a biological sample obtained from the subject;
processing the flow cytometry data to determine cell composition percentages for at least 20 cell types listed in Table 1;
generating a leukocyte signature for the subject using the determined cell composition percentages for the at least 20 cell types, the leukocyte signature comprising the cell composition percentages for the at least 20 cell types;
and
identifying, using the leukocyte signature and from among a plurality of leukocyte immunoprofile types, a leukocyte immunoprofile type for the subject.

3 more elements

Dependencies are inferred from the claim text.

Comprehensive immunoprofiling of peripheral blood

Life of this patent

Comprehensive immunoprofiling of peripheral blood

Life of this patent

Abstract

Claims30