Methods for assessing cellular or tissue injury using mitochondrial and/or nuclear cell-free dna
OwnerMichael Mitchell, Aoy Tomita Mitchellinventors
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Abstract
This invention relates to methods and compositions for assessing or monitoring an amount of mitochondrial cell-free DNA and/or nuclear cell-free DNA from a subject to assess cellular or tissue injury and/or risk in the subject. The amount(s) can be compared to specific threshold values, in some embodiments, to assess the subject and/or guide treatment of the subject.
Claims34
A method of assessing a sample from a subject that has or is suspected of having cellular or tissue injury, the method comprising:
- (a) determining an amount of mitochondrial cell-free DNA (mitochondrial cf-DNA) and/or nuclear cf-DNA in a sample taken from the subject;
- and
- (b) optionally, reporting and/or recording the amount of mitochondrial cf-DNA and/or nuclear cf-DNA.
A method of assessing a subject, the method comprising:
- (a) obtaining an amount of mitochondrial cell-free DNA (mitochondrial cf-DNA) and/or nuclear cf-DNA;
- and
- (b) comparing the amount of mitochondrial cf-DNA and/or nuclear cf-DNA to a threshold value, respectively, wherein when the amount is greater than or equal to the threshold value(s), risk is indicated;
- and
- (c) optionally, determining a treatment or monitoring regimen for the subject based on the comparison(s).
A method of assessing a sample from a subject that has undergone, is undergoing, or will undergo cellular or tissue injury or treatment for cellular or tissue injury, the method comprising:
- (a) determining an amount of mitochondrial cell-free DNA (mitochondrial cf-DNA) and/or nuclear cf-DNA in at least one sample taken from the subject, wherein the at least one sample is taken prior to cellular or tissue injury and/or taken post cellular or tissue injury;
- or
- (b) determining an amount of mitochondrial cf-DNA and/or nuclear cf-DNA in at least one sample taken from the subject, wherein the at least one sample is taken prior to treatment and/or taken post treatment;
- and
- (c) comparing the amount(s) to a threshold to assess a risk in the subject, wherein when the amount(s) are greater than or equal to the threshold value risk is indicated or increased.
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Dependencies are inferred from the claim text.