Cell culture device sensor caps, systems, and methods

A cell culture media monitoring system configured to non-invasively monitor cell culture media, the system comprising:

• a cell culture vessel comprising a cell culture chamber, the cell culture chamber having a surface on which cells are cultured;
• and
• a sensor cap configured to attach to the cell culture vessel, the sensor cap comprising:
• a cylindrical body having a closed end and an open end, wherein the open end is configured to attach to a port on the cell culture vessel;
• a sample collection chamber disposed on an interior surface of the cylindrical body;
• and
• a plurality of sensors in communication with the sample collection chamber.

A method of measuring cell culture media conditions comprising:

• attaching a sensor cap to a cell culture device, wherein the cell culture device comprises a cell culture surface for culturing cells and a volume of cell culture media:
• tilting the cell culture device to flow cell culture media to the sensor cap, wherein a sample of the cell culture media is collected in a sample collection chamber in the sensor cap;
• and
• measuring cell culture media conditions of the sample via a plurality of sensors in the sensor cap.