Fluid sampling system for biotechnological applications, operating method and use thereof

A fluid sampling system for biotechnological applications, comprising a bioreactor chamber for a fluid culture medium containing

• cells and further comprising
• transfer means for transferring controlled amounts of the fluid culture medium from said bioreactor chamber to a target container, wherein
• the transfer means comprise(s)
• a perfusion probe with a fluid-tight probe housing surrounding an internal probe volume and having an inlet probe aperture and an outlet probe aperture;
• a fluid filtering element sealingly connected to the fluid-tight probe housing and forming a cover of the inlet probe aperture, the fluid filtering element being formed as at least one monolithic platelet with a primary face and a secondary face opposed thereto,
• the primary face being in contact with the fluid culture medium when the perfusion probe is inserted into the bioreactor chamber or connected to the bioreactor chamber, and
• the secondary face being in contact with the internal probe volume of the perfusion probe, the fluid filtering element comprising an array of microchannels defining a filtering passage between the primary face and the secondary face, the microchannels each having a predetermined opening selected in a range of 0.2 to 64 μm;
• fluid driver means for driving the fluid culture medium from the bioreactor chamber through the perfusion probe to yield filtered culture medium, and for driving said filtered culture medium through a fluid connection line into said target container;
• wherein the fluid connection line comprises a fluid receptacle disposed between the outlet probe aperture of the perfusion probe and the target container,
• wherein
• the fluid receptacle is disposed on a weight measuring station configured for acquisition of a weight signal corresponding to a momentary weight of the fluid receptacle.