Genetically engineered swine for preclinical validation

A method of creating a genetically modified swine comprising a genetic modification in a Presenilin 1 (PSEN1) gene and a genetic modification in an amyloid beta precursor protein (APP) gene, the method comprising:

• introducing a guide RNA targeting a PSEN1 gene into a zygote to introduce double strand breaks in the PSEN1 gene to result in a deletion in the PSEN1 gene;
• introducing a guide RNA targeting an APP gene into the zygote to introduce double strand breaks in the APP gene to result in a deletion in the APP gene;
• culturing the zygote;
• transferring the cultured zygote to a recipient female;
• and allowing the zygote to develop in the recipient female to produce the genetically modified swine.

A method of creating a genetically modified swine comprising a deletion in a Presenilin 1 (PSEN1) gene, the method comprising:

• introducing a guide RNA targeting a PSEN1 gene into a zygote to introduce double strand breaks in the PSEN1 gene to result in a deletion in the PSEN1 gene;
• culturing the zygote;
• transferring the cultured zygote to a recipient female;
• and allowing the zygote to develop in the recipient female to produce the genetically modified swine.

A method of creating a genetically modified swine comprising a genetically modified swine comprising a deletion in an amyloid beta precursor protein (APP) gene, the method comprising:

• introducing a guide RNA targeting an APP gene into the zygote to introduce double strand breaks in the APP gene to result in a deletion in the APP gene;
• culturing the zygote;
• transferring the cultured zygote to a recipient female;
• and allowing the zygote to develop in the recipient female to produce the genetically modified swine.