Oxygen scavengers for electrochemical biosensors
AssigneeMaria Gabriela Machado de Almeida
InventorsMaria Gabriela Machado de Almeida, Tiago Carvalho Monteiro
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Abstract
A method of performing an electrochemical assay, comprising: reducing oxygen interference by introducing a biochemical oxygen scavenger to reduce dissolved oxygen directly into water.
Claims29
A method of performing an electrochemical assay, comprising:
- reducing oxygen interference by introducing a biochemical oxygen scavenger to reduce dissolved oxygen directly into water;
- wherein said biochemical oxygen scavenger is a single enzyme oxygen scavenger.
- wherein said single enzyme oxygen scavenger is a multicopper oxidase (MCO) enzyme oxygen scavenger.
- wherein said MCO enzyme oxygen scavenger comprises at least one of an ascorbate oxidase (AOx), or a bilirubin oxidase (BOD).
- wherein ascorbate is immobilized around but not directly on the working electrode containing reductase enzyme.
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The method of claim 13, wherein the nitrite reductase is cytochrome c nitrite reductase (ccNiR).
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The method of claim 19, wherein a sample applied to a microfluidic channel contacts the MCO oxygen scavenger before reaching a working electrode.
An electrochemical biosensor comprising a multicopper enzyme oxygen scavenger, an electrochemical assay containing a reductase enzyme, and a substrate comprising ascorbate;
- wherein the ascorbate is immobilized in a dried state and is not exposed to oxygen or light.
The electrochemical biosensor of claim 22, wherein the multicopper enzyme oxygen scavenger is one of ascorbate oxidase or bilirubin oxidase.
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32-42 (canceled).
Dependencies are inferred from the claim text.