Recombinant microorganism and method for producing c6 compound

A recombinant protein:

• (a) having an enzymatic activity to reduce 2,3-dehydroadipyl-CoA for conversion into adipyl-CoA, and
• (b) satisfying any one of the followings:
• (i) consisting of an amino acid sequence having a sequence identity of 80% or higher with an amino acid sequence set forth in SEQ ID NO:
• 8, 9, 10, 11, 12, 13, 14, 15, or 16;
• (ii) consisting of an amino acid sequence in which 1 to 10 amino acids are deleted, substituted, inserted, and/or added in an amino acid sequence set forth in SEQ ID NO:
• 8, 9, 10, 11, 12, 13, 14, 15, or 16;
• (iii) encoded by a DNA consisting of a polynucleotide sequence having a sequence identity of 80% or higher with a polynucleotide sequence set forth in SEQ ID NO:
• 57, 58, 59, 60, 61, 62, 63, 64, or 65;
• (iv) consisting of an amino acid sequence in which 1 to 10 amino acids are deleted, substituted, inserted, and/or added in an amino acid sequence of a protein encoded by a polynucleotide sequence set forth in SEQ ID NO:
• 57, 58, 59, 60, 61, 62, 63, 64, or 65;
• (v) encoded by a DNA hybridizing with a DNA consisting of a polynucleotide sequence complementary to a polynucleotide sequence set forth in SEQ ID NO:
• 57, 58, 59, 60, 61, 62, 63, 64, or 65 under a stringent condition;
• and
• (vi) encoded by a DNA consisting of a degenerate isomer of a polynucleotide sequence set forth in SEQ ID NO:
• 57, 58, 59, 60, 61, 62, 63, 64, or 65.

A recombinant protein:

• (xi) consisting of an amino acid sequence set forth in SEQ ID NO:
• 8, 9, 10, 11, 12, 13, 14, 15, or 16;
• or
• (xiii) encoded by a DNA consisting of a polynucleotide sequence set forth in SEQ ID NO:
• 57, 58, 59, 60, 61, 62, 63, 64, or 65.

(A-1) A recombinant polypeptide encoded by a DNA having a sequence identity of 80% or higher, 85% or higher, 88% or higher, 90% or higher, 93% or higher, 95% or higher, 97% or higher, 98% or higher, or 99% or higher with a PCR amplification product obtained using a chromosomal DNA of the genus Burkholderia (Burkholderia sp.) LEBP-3 strain as a template, and using, as a primer,

• (a1) a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 168 and a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 169, or
• (a2) a nucleotide having a base sequence set forth in SEQ ID NO:
• 168 and a nucleotide having a base sequence set forth in SEQ ID NO:
• 169,
• wherein the recombinant polypeptide has a 3-hydroxyadipyl-CoA dehydratase activity, and
• the DNA has 626 to 940 bp;
• (A-2) a recombinant polypeptide consisting of an amino acid sequence in which 1 to 10 amino acids are deleted, substituted, inserted, and/or added in an amino acid sequence of a polypeptide encoded by a DNA that is a PCR amplification product obtained using a chromosomal DNA of the genus Burkholderia (Burkholderia sp.) LEBP-3 strain as a template, and using, as a primer,
• (a1) a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 168 and a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 169, or
• (a2) a nucleotide having a base sequence set forth in SEQ ID NO:
• 168 and a nucleotide having a base sequence set forth in SEQ ID NO:
• 169,
• wherein the recombinant polypeptide has a 3-hydroxyadipyl-CoA dehydratase activity, and
• the DNA has 626 to 940 bp;
• or
• (A-3) a recombinant polypeptide encoded by a DNA consisting of a degenerate isomer of a PCR amplification product obtained using a chromosomal DNA of the genus Burkholderia (Burkholderia sp.) LEBP-3 strain as a template, and using, as a primer,
• (a1) a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 168 and a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 169, or
• (a2) a nucleotide having a base sequence set forth in SEQ ID NO:
• 168 and a nucleotide having a base sequence set forth in SEQ ID NO:
• 169,
• wherein the recombinant polypeptide has a 3-hydroxyadipyl-CoA dehydratase activity, and
• the DNA has 626 to 940 bp.

(B-1) A recombinant polypeptide encoded by a DNA having a sequence identity of 80% or higher, 85% or higher, 88% or higher, 90% or higher, 93% or higher, 95% or higher, 97% or higher, 98% or higher, or 99% or higher with a PCR amplification product obtained using a chromosomal DNA of the genus Burkholderia (Burkholderia sp.) LEBP-3 strain as a template, and using, as a primer,

• (b1) a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 170 and a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 171, or
• (b2) a nucleotide having a base sequence set forth in SEQ ID NO:
• 170 and a nucleotide having a base sequence set forth in SEQ ID NO:
• 171,
• wherein the recombinant polypeptide has a 2,3-dehydroadipyl-CoA reductase activity, and
• the DNA has 924 to 1,386 bp;
• (B-2) a recombinant polypeptide consisting of an amino acid sequence in which 1 to 10 amino acids are deleted, substituted, inserted, and/or added in an amino acid sequence of a polypeptide encoded by a DNA that is a PCR amplification product obtained using a chromosomal DNA of the genus Burkholderia (Burkholderia sp.) LEBP-3 strain as a template, and using, as a primer,
• (b1) a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 170 and a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 171, or
• (b2) a nucleotide having a base sequence set forth in SEQ ID NO:
• 170 and a nucleotide having a base sequence set forth in SEQ ID NO:
• 171,
• wherein the recombinant polypeptide has a 2,3-dehydroadipyl-CoA reductase activity, and
• the DNA has 924 to 1,386 bp;
• or
• (B-3) a recombinant polypeptide encoded by a DNA consisting of a degenerate isomer of a PCR amplification product obtained using a chromosomal DNA of the genus Burkholderia (Burkholderia sp.) LEBP-3 strain as a template, and using, as a primer,
• (b1) a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 170 and a nucleotide consisting of a base sequence set forth in SEQ ID NO:
• 171, or
• (b2) a nucleotide having a base sequence set forth in SEQ ID NO:
• 170 and a nucleotide having a base sequence set forth in SEQ ID NO:
• 171,
• wherein the recombinant polypeptide has a 2,3-dehydroadipyl-CoA reductase activity, and
• the DNA has 924 to 1,386 bp.