FLUORESCENT COMPOUND COMPRISING A FLUOROPHORE CONJUGATED TO A pH-TRIGGERED POLYPEPTIDE
AssigneesUniversity of Rhode Island Board of Trustees, Yale University
InventorsYana K. RESHETNYAK, Oleg A. ANDREEV, Donald M. ENGELMAN
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Abstract
The present subject matter provides compounds, compositions, and methods for identifying, monitoring, treating, and removing diseased tissue. Compounds, compositions, and methods for identifying, monitoring, and detecting circulating fluids such as blood are also provided.
Claims12
1-44. (canceled)
A method for detecting movement of a bodily fluid in a subject, comprising
- (a) administering a pH-triggered polypeptide fluorophore composition comprising the structure:
- to the subject;
- (b) contacting the subject with electromagnetic radiation comprising an excitation wavelength of the fluorophore;
- and
- (c) detecting electromagnetic radiation emitted from the composition, wherein detection of the radiation indicates the presence of the bodily fluid.
The method of claim 40, wherein the composition is administered to the subject via intravesical instillation, intravenous administration, intraperitoneal administration, topical administration, mucosal administration, oral administration, intraarterial administration, intracerebral administration, intracerebroventricular administration, intrathecal administration, intracardiac administration, intracavernous administration, intraosseous administration, intraocular administration, intravitreal administration, intramuscular administration, intradermal administration, transdermal administration, transmucosal administration, intralesional administration, subcutaneous administration, epicutaneous administration, extra-amniotic administration, intravaginal administration, intravesical administration, or nasal administration.
50-54. (canceled)
A method for detecting a fluorophore in a biological sample ex vivo,
- (a) contacting a biological sample from a subject with a pH-triggered polypeptide fluorophore composition comprising the structure:
- (b) contacting the biological sample with electromagnetic radiation comprising an excitation wavelength of the fluorophore;
- and
- (c) detecting electromagnetic radiation emitted from the composition.
The method of claim 58, wherein the biological sample comprises a tissue biopsy specimen, a liquid biopsy specimen, surgically removed tissue, a surgically removed liquid, or blood.
Dependencies are inferred from the claim text.