Loading patent…
AssigneeZYMtronix Catalytic Systems, Inc.
InventorsStephane Cedric Corgie, Alexander Chris Hoepker
Estimating the term…
The invention provides modular cell-free de-novo synthesis of glycans with immobilized bionanocatalysts. The invention provides materials, and in particular, magnetic materials, for producing glycans of defined length and sequences using one or more enzymes that are immobilized within bionanocatalysts (BNCs) which in turn are embedded within scaffolds to control the synthesis in batch or continuous processes manufacturing. In some embodiments, the scaffolds are high magnetism and high porosity composite blends of thermoplastics or thermosets comprising magnetic particles that form powders. In some embodiments, Selective Laser Sintering (SLS) is used to design and produce objects via 3D printing by sintering composite magnetic powders. The modular flow cells may be mixed and matched for a highly customizable and highly efficient cell-free manufacturing process. In some embodiments the elementary and system modules provided by the invention are employed. In preferred embodiments, human milk oligosaccharides (HMOs) are produced.
A module comprising a magnetic macroporous matrix material comprising self-assembled mesoporous aggregates of magnetic nanoparticles magnetically entrapping an immobilized glycan synthesis enzyme.
-35. (canceled)
-40. (canceled)
A method for making a glycan, comprising the steps of
-54. (canceled)
-60. (canceled)
A method of making a device for catalyzing an enzymatic reaction, comprising combining a magnetic macroporous scaffold with self-assembled mesoporous aggregates of magnetic nanoparticles and a glycan synthesis enzyme, wherein said enzyme is magnetically immobilized within or in said mesopores.
A modular process for producing a glycan, comprising a module wherein:
A process for preparing a scaffolded bionanocatalyst by combining a magnetic nanoparticle and a glycan synthesis enzyme to form a bionanocatalyst and then contacting the bionanocatalyst with a scaffold to obtain the scaffolded bionanocatalyst.
A process for preparing a scaffolded bionanocatalyst by combining a scaffold and a magnetic nanoparticle to form a scaffolded magnetic nanoparticle complex and then contacting the scaffolded magnetic nanoparticle complex with a glycan synthesis enzyme.
A scaffolded bionanocatalyst comprising a magnetic macroporous matrix material comprising self-assembled mesoporous aggregates of magnetic nanoparticles magnetically entrapping an immobilized glycan synthesis enzyme.
-68. (canceled)
-73. (canceled)
Dependencies are inferred from the claim text.