Storage-Stable Hydrolase Containing Liquids

A liquid detergent formulation comprising at least component (a):

• at least one enzyme selected from the group of hydrolases (EC 3);
• component (b):
• an enzyme-stabilizing system comprising
• (bi) at least one compound according to general formula (A)
• wherein the variables in formula (A) are as follows:
• R1 is selected from the group consisting of H and C1-C10 alkylcarbonyl, wherein alkyl may be linear or branched and may bear one or more hydroxyl groups,
• R2, R3, R4 are independently from each other selected from the group consisting of H, linear C1-C5 alkyl, branched C3-C10 alkyl, C6-C10-aryl, non-substituted or substituted with one or more carboxylate or hydroxyl groups, and C6-C10-aryl-alkyl, wherein alkyl of the latter is selected from the group consisting of linear C1-C8 alkyl and branched C3-C8 alkyl, wherein at least one of R2, R3, and R4 is not H;
• and
• (bii) at least one compound selected from the group consisting of boron containing compound and peptide stabilizer,
• and
• component (c):
• at least one diol having terminal —OH groups containing 4 to 8 C-atoms and at least one detergent component.

A method of using at least one diol having terminal —OH groups containing 4 to 8 C-atoms, the method comprising using the at least one diol to provide homogenous and storage-stable enzyme preparations comprising at least

• component (a):
• at least one enzyme selected from the group of hydrolases (EC 3);
• and
• component (b):
• an enzyme-stabilizing system comprising
• (bi) at least one compound according to general formula (A)
• wherein the variables in formula (A) are as follows:
• R1 is selected from the group consisting of H and C1-C10 alkylcarbonyl, wherein alkyl may be linear or branched and may bear one or more hydroxyl groups,
• R2, R3, R4 are independently from each other selected from the group consisting of H, linear C1-C5 alkyl, branched C3-C10 alkyl, C6-C10-aryl, non-substituted or substituted with one or more carboxylate or hydroxyl groups, and C6-C10-aryl-alkyl, wherein alkyl of the latter is selected from the group consisting of linear C1-C8 alkyl and branched C3-C8 alkyl, wherein at least one of R2, R3, and R4 is not H;
• and
• (bii) at least one compound selected from the group consisting of boron containing compound and peptide stabilizer.

A method of using at least one diol selected from the group consisting of diols having terminal —OH groups containing 3 to 10 C-atoms, the method comprising using the at least one diol to improve

• enzyme stability of at least one hydrolase and/or
• enzyme preparation stability
• in the presence of a compound according to according to general formula (A)
• wherein the variables in formula (A) are as follows:
• R1 is selected from the group consisting of H and C1-C10 alkylcarbonyl, wherein alkyl may be linear or branched and may bear one or more hydroxyl groups,
• R2, R3, R4 are independently from each other selected from the group consisting of H, linear C1-C5 alkyl, branched C3-C10 alkyl, C6-C10-aryl, non-substituted or substituted with one or more carboxylate or hydroxyl groups, and C6-C10-aryl-alkyl, wherein alkyl of the latter is selected from the group consisting of linear C1-C8 alkyl and branched C3-C8 alkyl, wherein at least one of R2, R3, and R4 is not H.