US 2024/0123058 A1Published application

Epitope peptide and antibody for preventing and treating eb virus infection and related diseases

AssigneesXiamen University, Sun Yat-Sen University Cancer Center (Sun Yat-Sen University Cancer Center Hospital, Sun Yat-Sen, Sun Yat-Sen University

InventorsYixin CHEN, Xiao ZHANG, Junping HONG, Miao XU, Qian WU, Ling ZHONG, Ningshao XIA

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Abstract

Provided are an epitope peptide (or a variant thereof) that can be used for preventing or treating an EBV infection, a recombinant protein containing the epitope peptide (or variant thereof) and a carrier protein, and the use of the epitope peptide (or variant thereof) and the recombinant protein. Further provided are an antibody against the epitope peptide, and the use thereof in the detection, prevention and/or treatment of an EBV infection and/or diseases caused by the infection.

Claims42

Dependent claims

An antibody or antigen-binding fragment thereof, which specifically binds to an epitope comprised in amino acid residues at positions 341-362 of an EBV gB protein, wherein the epitope comprises at least amino acid residues at positions 352, 356 and 360 of the EBV gB protein;

preferably, the epitope is a linear epitope, which consists of at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15 or at least 20 consecutive amino acid residues located within the amino acid residues at positions 341-362 of EBV gB protein;
preferably, the epitope is a conformational epitope, which consists of at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15 or at least 20 non-consecutive amino acid residues located within the amino acid residues at positions 341-362 of EBV gB protein, and comprises at least amino acid residues at positions 352, 356 and 360 of EBV gB protein;
preferably, the epitope consists of the amino acid residues at positions 341-362 of EBV gB protein.

An antibody or antigen-binding fragment thereof, which specifically binds to a gB protein of EBV, wherein the antibody or antigen-binding fragment thereof comprises:

(a) a heavy chain variable region (VH) comprising the following 3 complementarity determining regions (CDRs):
(i) a VH CDR1, which consists of the following sequence:
SEQ ID NO:
3, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto,
(ii) a VH CDR2, which consists of the following sequence:
SEQ ID NO:
4, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto, and
(iii) a VH CDR3, which consists of the following sequence:
SEQ ID NO:
5, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto;
and/or,
(b) a light chain variable region (VL) comprising the following 3 complementarity determining regions (CDRs):
(iv) a VL CDR1, which consists of the following sequence:
SEQ ID NO:
6, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto,
(v) a VL CDR2, which consists of the following sequence:
SEQ ID NO:
7, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto, and
(vi) a VL CDR3, which consists of the following sequence:
SEQ ID NO:
8, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto;
preferably, the CDR described in any one of (i) to (vi) is defined according to the IMGT numbering system;
preferably, the substitution described in any one of (i) to (vi) is a conservative substitution.

20 more elements

An antibody or antigen-binding fragment thereof, which specifically binds to an epitope comprised in amino acid residues at positions 528-616 of an EBV gB protein, wherein the epitope comprises at least amino acid residues at positions 540, 567, 610 and 613 of the EBV gB protein;

preferably, the epitope is a linear epitope, which consists of at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15 or at least 20 consecutive amino acid residues located within the amino acid residues at positions 528-616 of EBV gB protein;
preferably, the epitope is a conformational epitope, which consists of at least 3, at least 4, at least 5, at least 6, at least 7, at least Consists of 8, at least 9, at least 10, at least 15 or at least 20 non-consecutive amino acid residues located within the amino acid residues at positions 528-616 of EBV gB protein, and comprises at least amino acid residues at positions 540, 567, 610 and 613 of EBV gB protein;
preferably, the epitope consists of amino acid residues at positions 528-616 of EBV gB protein.

An antibody or antigen-binding fragment thereof, which specifically binds to a gB protein of EBV, wherein the antibody or an antigen-binding fragment thereof comprises:

(a) a heavy chain variable region (VH) comprising the following 3 complementarity determining regions (CDRs):
(i) a VH CDR1, which consists of the following sequence:
SEQ ID NO:
11, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto,
(ii) a VH CDR2, which consists of the following sequence:
SEQ ID NO:
12, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto, and
(iii) a VH CDR3, which consists of the following sequence:
SEQ ID NO:
13, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto;
and/or,
(b) a light chain variable region (VL) comprising the following 3 complementarity determining regions (CDRs):
(iv) a VL CDR1, which consists of the following sequence:
SEQ ID NO:
14, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto,
(v) a VL CDR2, which consists of the following sequence:
SEQ ID NO:
15, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto, and
(vi) a VL CDR3, which consists of the following sequence:
SEQ ID NO:
16, or a sequence having a substitution, deletion or addition of one or several amino acids (e.g., a substitution, deletion or addition of 1, 2 or 3 amino acids) as compared thereto;
preferably, the CDR described in any one of (i) to (vi) is defined according to the IMGT numbering system;
preferably, the substitution described in any one of (i) to (vi) is a conservative substitution.

20 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/276,700Filed 29 Jan 2022
Priority: CN 202110182104.910 Feb 2021
Published: 18 Apr 2024
Term ends: Not estimatedThe source records don’t support an estimate.

Classification29 codes

A61K 39/245
Viral antigens: herpetoviridae, e.g. herpes simplex virus
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
A61K 39/12
Medicinal preparations containing antigens or antibodies: viral antigens
A61P 31/20
Antivirals: for DNA viruses

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