Method for detecting ammonia nitrogen content by using nitrification biological reaction

A method for detecting ammonia nitrogen content by a nitrification biological reaction, comprising the following steps:

• S1. culture and acclimation of nitrification microorganism membrane, comprising:
• continuously conveying an environmental water sample with a temperature of 10-45° C. and a nitrification nutrient solution to a surface of a substrate until a nitrification microorganism membrane is formed on the surface of the substrate,
• wherein the nitrification nutrient solution is a mono-component or multi-component solution containing inorganic nitrogen and inorganic carbon;
• and
• S2. ammonia nitrogen detection:
• S21. standard curve plotting, comprising:
• respectively flowing an ammonia-free water sample and an ammonia-containing standard water sample through the nitrification microorganism membrane obtained in step S1;
• measuring a dissolved oxygen concentration of an effluent ammonia-free water sample and a dissolved oxygen concentration of an ammonia-containing standard water sample, wherein the dissolved oxygen concentration of the effluent ammonia-free water sample is recorded as DO1, and the dissolved oxygen concentration of the effluent ammonia-containing standard water sample is recorded as DO2;
• calculating a difference value of dissolved oxygen concentration, ΔDO, between the two effluent water samples;
• and obtaining a fitting formula according to the ΔDO and an ammonia nitrogen concentration of the ammonia-containing standard water sample;
• and
• S22. concentration calculation, comprising:
• respectively flowing an ammonia-free water sample and an ammonia-containing water sample to be detected through the nitrification microorganism membrane obtained in step S1, detecting and calculating to obtain a difference value of dissolved oxygen concentration, ΔDO, between the two effluent water samples, and substituting the difference value into the fitting formula obtained in step S21 to calculate an ammonia nitrogen concentration of the ammonia-containing water sample to be detected.