Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof

A recombinant bacterial cell comprising:

• three copies of a methionine decarboxylase (metDC) gene, wherein each copy of the metDC gene is operably linked to a separate IPTG inducible promoter, and wherein the metDC gene encodes a polypeptide comprising SEQ ID NO:
• 1048;
• a metP gene operably linked to an IPTG inducible promoter, wherein the metP gene encodes a polypeptide comprising SEQ ID NO:
• 1056;
• a knock-out of an endogenous methionine efflux pump, wherein the endogenous methionine efflux pump is encoded by a yjeH gene, and wherein the yjeH gene comprises a sequence comprising SEQ ID NO:
• 1014;
• a ΔdapA auxotrophy;
• a modified endogenous colibactin island, wherein the modified endogenous colibactin island comprises a deletion of clbA (SEQ ID NO:
• 1065), clbB (SEQ ID NO:
• 1066), clbC (SEQ ID NO:
• 1067), clbD (SEQ ID NO:
• 1068), clbE (SEQ ID NO:
• 1069), clbF (SEQ ID NO:
• 1070), clbG (SEQ ID NO:
• 1071), clbH (SEQ ID NO:
• 1072), clbI (SEQ ID NO:
• 1073), clbJ (SEQ ID NO:
• 1074), clbK (SEQ ID NO:
• 1075), clbL (SEQ ID NO:
• 1076), clbM (SEQ ID NO:
• 1077), clbN (SEQ ID NO:
• 1078), clbO (SEQ ID NO:
• 1079), clbP (SEQ ID NO:
• 1080), clbQ (SEQ ID NO:
• 1081), and/or clbR (SEQ ID NO:
• 1082);
• and
• a deletion of an endogenous phage gene, wherein the endogenous phage gene comprises a sequence comprising SEQ ID NO:
• 1064.