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AssigneeNational University of Singapore
InventorsKevin Jie Han Lim, Maybelle Darlene Kho Go, Wen Shan Yew
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A recombinant cell of Saccharomyces cerevisiae that includes in its genome nucleic acids encoding cannabinoid biosynthetic pathway genes. A cannabinoid is produced by the recombinant cell in the presence of a cannabinoid precursor substrate and at least one of the cannabinoid biosynthetic pathway genes is from an organism other than Cannabis sativa, wherein the at least one of the cannabinoid biosynthetic pathway genes encodes a prenyltransferase. In an embodiment, the prenyltransferase is NphB from Streptomyces sp. having the amino acid sequence of any one of SEQ ID NOs: 8-11. Also disclosed is a method for producing a cannabinoid with the recombinant cell and the cannabinoid precursor substrate.
A recombinant cell of Saccharomyces cerevisiae, comprising in its genome a plurality of nucleic acids each encoding a cannabinoid biosynthetic pathway gene, wherein a cannabinoid is produced by the recombinant cell in the presence of a cannabinoid precursor substrate and at least one of the cannabinoid biosynthetic pathway genes is from an organism other than Cannabis sativa.
A prenyltransferase having the amino acid sequence of any one of SEQ ID NOs:
A nucleic acid encoding a prenyltransferase having the amino acid sequence of any one of SEQ ID NOs:
Dependencies are inferred from the claim text.