Microspheres Containing Decellularized Donor Tissue and Their Use in Fabricating Polymeric Structures

A method of treating a subject suffering from a tissue injury, the method comprising applying to the injury a fabricated filament comprising:

• a plurality of decellularized matrix microspheres comprising a first polymer encapsulating a decellularized donor tissue, wherein the first polymer comprises one or more of polycaprolactone (PCL), polyvinyl acetate (PVAC), ethylene vinyl acetate polymer (EVA), polyvinyl alcohol (PVA), polylactic acid (PLA), polyglycolic acid (PGA), polylactic-co-glycolic acid (PLGA), polyalkyl cyanoacrylate, polyurethane, and nylons;
• and
• a second polymer,
• wherein the decellularized matrix microspheres and the second polymer are co-extruded in the form of the filament.