Barley with very low levels of hordeins
AssigneesCommonweath Scientific and Industrial Research Organisation, Grains Research and Development Corporation
InventorsGregory John Tanner, Crispin Alexander Howitt, Michelle Lisa Colgrave, Malcolm James Blundell
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Abstract
The present invention relates to methods of producing a food or malt-based beverage suitable for consumption by a subject with Coeliac's disease. In particular, the present invention relates to methods of producing a food or malt-based beverage with very low levels of hordeins. Also provided are barley plants which produce grain that can be used in the methods of the invention.
Claims58
A method of producing a food or malt-based beverage ingredient, or a food or a malt-based beverage, the method comprising (i) processing barley grain to produce malt, wort, flour or wholemeal, and/or (ii) mixing barley grain, or malt, wort, flour or wholemeal produced from said grain, with at least one other food or beverage ingredient, wherein the barley grain, malt, wort, flour or wholemeal comprises about 50 ppm or less hordeins, thereby producing the food or malt-based beverage ingredient, food or malt-based beverage.
A barley plant which produces grain comprising about 50 ppm or less hordeins.
Beer comprising one or more barley grain proteins and less than 0.9 ppm hordeins.
Flour or wholemeal comprising one or more barley grain proteins and less than about 50 ppm, or less than about 20 ppm, hordeins.
Malt or wort comprising one or more barley grain proteins and less than about 50 ppm, or less than about 20 ppm, hordeins.
A method of identifying an allele of a barley D hordein gene which encodes a truncated D hordein, the method comprising
- i) obtaining a sample comprising nucleic acids from a barley plant, and
- ii) analysing the sample for the presence or absence of a guanine residue at position 450 of the open reading frame encoding D hordein,
- wherein the presence of the guanine indicates the D hordein gene encodes a truncated D hordein.
Dependencies are inferred from the claim text.