Packing assembly for chromatography media and methods of use thereof

A method of packing chromatography media in a packing assembly to form a packed-bed, the method comprising:

• a) placing the chromatography media in the packing assembly when the packing assembly is in an assembled state, the packing assembly comprising
• a bottom plate;
• a first top plate defining a top plate hole centrally positioned in the first top plate;
• a middle plate defining a middle plate hole centrally positioned in the middle plate, the middle plate hole being aligned with the top plate hole when the packing assembly is in the assembled state where the middle plate is coupled to each of the bottom plate and the first top plate and the middle plate is positioned between the bottom plate and the first top plate;
• and
• a second top plate having a protrusion extending outwardly, at least a portion of the protrusion having a same size and a same shape as the top plate hole to be received in the top plate hole when the packing assembly is in a packing state;
• b) inserting the protrusion of the second top plate into the top plate hole;
• and
• c) applying a force to the second top plate to put the packing assembly into the packing state and compress the chromatography media into the middle plate hole to form the packed-bed in the middle plate.

A method of assembling a chromatography device, the method comprising:

• a) placing the chromatography media in the packing assembly, the packing assembly comprising
• a bottom plate;
• a middle plate defining a middle plate hole centrally positioned in the middle plate;
• a first top plate defining a top plate hole centrally positioned in the first top plate, the middle plate being aligned with the top plate hole when the packing assembly is in the assembled state where the middle plate is coupled to each of the bottom plate and the first top plate and the middle plate is positioned between the bottom plate and the first top plate;
• and
• a second top plate having a protrusion extending outwardly, at least a portion of the protrusion having a same size and same shape as the top plate hole to be received in the top plate hole when the packing assembly is in a packing state;
• b) inserting the protrusion of the second top plate into the top plate hole;
• c) applying a force to the second top plate to put the packing assembly into the packing state and compress the chromatography media in the middle plate hole to form the packed-bed in the middle plate;
• d) disassembling the packing assembly to separate the middle plate from the bottom plate and the first top plate;
• and
• e) placing the middle plate with the packed-bed in the chromatography device.