Coronavirus vaccine and method for preparation thereof

A method for preparation of killed-inactivated purified SARS-CoV2 bulk comprising the following steps:

• (a) using Vero cell line (with or without serum) as cell substrate for SARS-CoV-2 virus culture,
• (b) scaling up the SARS-CoV-2 virus culture of step (a) upto a harvest volume of 10L and above,
• (c) inactivating the SARS-CoV-2 virus culture of step (b), and
• (d) purifying the SARS-CoV-2 virus culture obtained in step (c) and obtaining the killed-inactivated purified SARS-CoV2 bulk.

A method of inactivation of SARS-CoV-2 virus selected from:

• i) Formalin treatment at any concentration ranging from 1:500 upto 1:
• 4000 v/v of formalin:
• virus, at 8° C. to 37° C., preferably 25±3° C., for at least 1 to 7 days;
• ii) Formalin treatment at any concentration ranging from 1:500 upto 1:
• 4000 v/v of formalin:
• virus, at 2° C. to 8° C. for at least 10 to 30 days;
• iii) Beta propiolactone at any concentration ranging from 1:500 upto 1:
• 4000 v/v of BPL:
• virus, for at least 24 to 48 hrs at temperatures ranging from 8° C. to 30° C., preferably 25±3° C., for 48 hrs;
• iv) Beta propiolactone at any concentration ranging from 1:500 upto 1:
• 4000 v/v of BPL:
• virus, at 2° C. to 8° C. for at least 3-7 days;
• v) A combination of BPL and formalin at any aforementioned conditions, preferably BPL inactivation at 1:
• 3000 (PBL:
• virus v/v) for 24 hrs followed by formalin inactivation at 1:3000 (formalin:virus, v/v) for 24 to 48 hrs at 15° C. to 30° C., preferably 25±3° C.