Time delay integration acquisition for spatial genomics imaging

A method of capturing spatial-omic images of a biological tissue sample, the method comprising:

• securing a biological tissue sample in an imaging chamber of an imaging system;
• directing light from a light source to illuminate an area on the biological tissue sample;
• and
• causing a Time Delay and Integration (TDI) imager to scan the biological tissue sample where the biological tissue sample is illuminated by the light the light source using one or more TDI scans of the biological tissue sample.

An imaging system for capturing spatial-omic images of biological tissue samples, the imaging system comprising:

• an imaging chamber configured to secure a biological tissue sample placed in the imaging system;
• a Time Delay and Integration (TDI) imager;
• a light source configured to illuminate an area on the biological tissue sample that is being captured by the TDI imager with a plurality of distinct wavelengths;
• and
• a controller configured to cause the TDI imager to simultaneously capture a plurality of distinct images of the biological tissue sample, wherein each of the plurality of distinct images corresponds to one of the plurality of distinct wavelengths.