Plant body of genus nicotiana and production method therefor
AssigneeJapan Tobacco Inc.
InventorsTakanori TAKEUCHI, Hisashi UDAGAWA, Yoshimitsu TAKAKURA, Hiroshi MAGOME, Ryo NISHIGUCHI
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Abstract
Provided are a tobacco plant in which a high solavetivone content can be achieved without inoculation with a virus, and a method for producing the tobacco plant. In a tobacco plant in accordance with an aspect of the present invention, a function of an endogenous CA1 gene is suppressed.
Claims20
A tobacco plant in which a mutation that causes suppression of a function of an endogenous gene is introduced in a genome, the endogenous gene being at least one of:
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 2;
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 4;
- and
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 6,
- where said tobacco plant is not Nicotiana benthamiana if the suppression of the function is promotion by RNAi or VIGS of degradation of mRNA which has been transcribed from the at least one of the endogenous genes, as compared with a wild-type plant.
A method of producing a tobacco plant,
- said method comprising the step of introducing, in a genome of the tobacco plant, a mutation that causes suppression of a function of an endogenous gene, the endogenous gene being at least one of:
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 2;
- an endogenous gene which contains, as a coding having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 4;
- and
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 6,
- where the tobacco plant is not Nicotiana benthamiana if the suppression of the function is promotion by RNAi or VIGS of degradation of mRNA which has been transcribed from the at least one of the endogenous genes, as compared with a wild-type plant.
A cured leaf of a tobacco plant in which a mutation that causes suppression of a function of an endogenous gene is introduced in a genome, the endogenous gene being at least one of:
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 2;
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 4;
- and
- an endogenous gene which contains, as a coding region, a polynucleotide that encodes a polypeptide having a sequence identity of 95% or higher with an amino acid sequence represented by SEQ ID NO:
- 6,
- where the tobacco plant is not Nicotiana benthamiana if the suppression of the function is promotion by RNAi or VIGS of degradation of mRNA which has been transcribed from the at least one of the endogenous genes, as compared with a wild-type plant,
- said cured leaf having a solavetivone content higher than that of a cured leaf of a wild-type tobacco plant.
Dependencies are inferred from the claim text.