Purification of sulfonated dna
AssigneeExact Sciences Corporation
InventorsEvan M. Ragland, Zubin Gagrat, Michael J. Domanico
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods and systems for purification of chemically modified, specifically, sulfonated DNA. Additionally, the disclosure provides methods and systems for bisulfite conversion with improved purification of sulfonated DNA using silica supports, such as silica beads, and acidic conditions for binding sulfonated DNA to silica surfaces.
Claims23
A method comprising combining sulfonated DNA and a silica support in an acidic binding solution, wherein the acidic binding solution has a pH less than or equal to an isoelectric point (pH(I)) of the sulfonated DNA, and wherein sulfonated DNA is bound to the silica support.
5-7. (canceled)
15-20. (canceled)
A composition comprising sulfonated DNA and a silica support in an acidic binding solution comprising a chaotropic salt, wherein the acidic binding solution has a pH less than or equal to a pH(I) of the sulfonated DNA, and wherein the sulfonated DNA is bound to the silica support.
27-31. (canceled)
A kit, comprising:
- i) a bisulfite reagent solution or components for preparing a bisulfite reagent solution;
- ii) a silica support;
- and
- iii) an acidic component having a pH less than or equal to a pH(I) of sulfonated DNA.
34 -67. (canceled)
Dependencies are inferred from the claim text.