Methods for Culturing Antibody Secreting Cells
OwnerShayne Guilianoinventor
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Abstract
The invention relates to methods for culturing antibody secreting cells, and related methods, constructs and compositions.
Claims35
A bioreactor construct comprising antibody secreting cells and a cell monolayer in contact with a surface of a plurality of hollow capillary tubes arranged in a parallel array within a tubular cartridge defining an intracapillary (IC) space and an extracapillary (EC) space of the bioreactor, each hollow capillary tube constructed of a semi-permeable membrane defining an internal surface adjacent to the IC space and an external surface adjacent to the EC space, wherein the cell monolayer is in contact with the internal or external surface of the plurality of capillary tubes and wherein a cell culture medium fills the IC space or the EC space, or both.
A method for culturing antibody secreting cells, the method comprising seeding a bioreactor construct with antibody secreting cells and a second type of cell selected from epithelial cells, fibroblast cells, or a combination thereof;
- wherein the bioreactor construct comprises a surface of a plurality of hollow capillary tubes arranged in a parallel array within a tubular cartridge defining an intracapillary (IC) space and an extracapillary (EC) space of the bioreactor, each hollow capillary tube constructed of a semi-permeable membrane defining an internal surface adjacent to the IC space and an external surface adjacent to the EC space;
- wherein the second cell type forms a monolayer in contact with the internal or external surface of the plurality of capillary tubes;
- and wherein a cell culture medium fills the IC space or the EC space, or both.
Dependencies are inferred from the claim text.