Compositions, methods and systems for protein corona analysis and uses thereof

A high-throughput method of preparing to identify proteins in a biological sample, the method comprising:

• (a) incubating magnetic microparticles with the biological sample to form protein coronas comprising proteins, the magnetic microparticles comprising:
• (i) silica, (ii) carboxylate functional groups, (iii) negative surface charges from −50 mV to −10 mV in zeta potential, and (iv) a heterogeneous distribution of sizes including from about 1 μm to about 10 μm in diameter, wherein the magnetic microparticles are configured to form protein coronas having at least 200 unique protein groups that can be identified using mass spectrometry;
• (b) magnetically isolating the magnetic microparticles, by applying an external magnetic field, from unbound protein in the biological sample to enrich the proteins in the protein coronas;
• (c) washing the enriched proteins and the magnetic microparticles after magnetically isolating the magnetic microparticles;
• (d) lysing the enriched proteins in the protein coronas by adding a lyse reagent to the protein coronas and the magnetic microparticles;
• (e) digesting the enriched proteins in the protein coronas to generate digested peptides by adding a digestion reagent to the protein coronas and the magnetic microparticles;
• and
• (f) purifying the digested peptides.