Tissue-mimicking hydrogel compositions for biofabrication
AssigneeWake Forest University Health Sciences
InventorsAleksander Skardal, Shay Soker
Life of this patent
Estimating the term…
Abstract
An extrudable hydrogel composition useful for making a three-dimensional organ construct includes a cross-linkable prepolymer, a post-deposition crosslinking group, optionally, an initiator that catalyzes the reaction between the prepolymer and said the crosslinking group; live cells (e.g., plant, animal, or microbial cells), optionally at least one growth factor, and optionally water to balance. Methods of using the same and products so made are also described.
Claims22
A method of making a three-dimensional organ construct,
- comprising the steps of:
- (a) providing a reservoir containing an extrudable hydrogel composition, said composition comprising:
- a cross-linkable prepolymer;
- a post-deposition crosslinking group;
- and
- live cells;
- then
- (b) depositing said hydrogel composition onto a substrate;
- and then
- (c) cross-linking said prepolymer with said post-deposition crosslinking group by an amount sufficient to increase the stiffness of said hydrogel and form said three-dimensional organ construct.
(canceled)
A device useful for modeling cellular function in vitro, comprising:
- (a) a microfluidic device substrate having at least one chamber formed therein;
- (b) a hydrogel composition deposited in said chamber in a first pattern,
- (c) live cells in said hydrogel composition;
- and
- (d) a structural support polymer deposited in said chamber adjacent said hydrogel.
-15. (canceled)
Dependencies are inferred from the claim text.