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US 2023/0417669 A1Published application
Gram-Stain Differentiation with Nonlinear Light Scattering
AssigneeTemple University of the Commonwealth System of Higher Education
InventorsHai-Lung Dai, Mohammad Sharifian, Michael Wilhelm
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Abstract
The present invention generally relates to improved methods for characterizing the cell wall complex composition of a bacterial specimen via differential transport kinetics of a small molecule. The present invention also relates to improved and automated methods for Gram differentiation of a bacterial specimen using nonlinear optical techniques.
Claims20
A method of determining bacterial cell wall composition comprising the steps of:
- a. measuring a first second-harmonic scattering (SHS) signal of a small molecule in a solution to generate a baseline;
- b. adding a bacterial specimen to said solution to generate a suspension;
- c. measuring a second SHS signal of said suspension to generate a response;
- and
- d. determining whether said response is above or below said baseline.
2 more elements
An imaging method of differentiating Gram-negative and Gram-positive bacteria comprising the steps of:
- a. obtaining an optical image of a sample expected to contain one or more strain of bacterium;
- b. adding CV to said sample;
- c. irradiating said sample with fundamental light;
- d. imaging second-harmonic scattering (SHS) light for at least 101 seconds;
- e. overlaying the integrated image intensity of said SHS image obtained >100 seconds after CV addition over said optical image of said sample;
- and
- f. identifying said bacterium that are overlaid with a positive SHS signal as Gram-negative and those that are not overlaid with a positive SHS signal as Gram-positive.
4 more elements
Dependencies are inferred from the claim text.