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US 2023/0416785 A1Published application
Nuclease and application thereof
AssigneeCRISP-HR Therapeutics, Inc.
InventorChristopher Hackley
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Abstract
Described herein are compositions comprising a nuclease. Also described herein are methods utilizing the compositions comprising the nuclease.
Claims33
A method for introducing an edit into a genomic locus of a plurality of cells, the method comprising:
- contacting the plurality of the cells with:
- a) a Cas fusion protein complex comprising a Cas fusion protein complexed with a guide polynucleotide configured to bind to the genomic locus of a cell of the plurality of cells;
- and
- b) a repair template comprising at least 5000 base pairs (bp) in length,
- thereby introducing the edit into the genomic locus of the plurality of cells with the repair template, wherein the edit comprises a homology directed repair (HDR).
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Dependencies are inferred from the claim text.