Methods and systems for mechanoporation-based high-throughput payload delivery into biological cells

A cell processing apparatus for processing cells using mechanoporation, the cell processing apparatus comprising:

• an inlet plate comprising an inlet opening;
• an outlet plate comprising an outlet opening;
• and
• a processing component stacked between the inlet plate and the outlet plate, wherein:
• the processing component comprises dividers, each comprising a first divider wall, a second divider wall, and a first surface, extending between the first divider wall and the second divider wall,
• the processing component further comprises a channel wall, ridges protruding from the channel wall, and a second surface,
• the first surface of the processing component directly contacts one of the inlet plate and the outlet plate,
• the first divider wall, the second divider wall, the channel wall of the processing component and the one of the inlet plate and the outlet plate define multiple channels, each fluidically coupled and extending between the inlet opening and the outlet opening and configured for flowing a combination of a cell media and the cells such that one or more of the ridges of the processing component extend into each of the multiple channels, and
• each of the ridges of the processing component forms a gap with the one of the inlet plate and the outlet plate such that the gap is smaller than a diameter of at least one of the cells thereby causing compression of the at least one of the cells while the cells pass through the gap.