Hairpin oligonucleotides and uses thereof

Abstract

In aspects, the invention provides a hairpin oligonucleotide comprising a 3′-terminal nucleotide, wherein the sugar component of the 3′-terminal nucleotide comprises a 2′-hydroxyl and a 3′-phosphate. In aspects, the invention provides a hairpin oligonucleotide comprising a 3′-terminal nucleotide wherein the sugar position of the 3′-terminal nucleotide comprises a 2′,3′-dialdehyde oxidation product of a sugar. In aspects, the invention provides use of a hairpin oligonucleotide in developing a biomarker. In aspects, the invention provides a solid support comprising a ligand moiety and a hairpin oligonucleotide, wherein the oligonucleotide is immobilized on the solid support through binding of the affinity moiety of the hairpin oligonucleotide to the ligand moiety of the solid support. In aspects, the invention also provides a method of preparing an RNA sequence library comprising: (a) ligating an RNA sequence to a hairpin oligonucleotide to form a construct, (b) reverse-transcribing the RNA sequence as a cDNA sequence, and (c) amplifying the cDNA sequence using PCR.

Dependencies are inferred from the claim text.