Integrated microfluidic system for the processing of tissues into cellular suspensions

A microfluidic system for processing a tissue sample comprising:

• a microfluidic digestion device comprising an inlet and an outlet and a flow path defined between the inlet and the outlet, the flow path comprising a tissue chamber configured to hold the tissue sample and a plurality of upstream fluidic channels communicating with the tissue chamber on the inlet side of the flow path and a plurality of downstream fluidic channels communicating with the tissue chamber on the outlet side of the flow path;
• a first pump configured to pump a buffer-containing fluid and/or an enzyme-containing fluid into the inlet of the microfluidic digestion device;
• a microfluidic dissociation/filter device comprising an inlet, a first outlet, a second outlet, and a flow path defined between the inlet and the outlet, the flow path comprising a plurality of furcating dissociation channels having a plurality of expansion and constriction regions disposed along a length thereof, wherein one or more filters are disposed in the flow path downstream of the plurality of furcating dissociation channels;
• a second pump configured to pump a buffer-containing or other fluid into the inlet of the microfluidic dissociation/filter device;
• and
• wherein the outlet of the microfluidic digestion device is fluidically coupled to the inlet of the microfluidic dissociation/filter device.