Method for manufacturing an analysis chip and analysis chip

Method for manufacturing a biological sample analysis chip, wherein the method comprises:

• a matrix formed in a solid support material, having a lower surface and an upper surface and in which at least one hole extending between said lower and upper surfaces has been formed, is provided, wherein said through hole is a cylinder of revolution;
• at least one pad is provided, cut from a sheet of solid and porous analysis material, said pad having a lower surface and an upper surface,
• the at least one pad is inserted into the at least one through hole of the matrix by translation of the at least one pad in the direction normal to the lower and upper surfaces of the matrix;
• a mechanical assembly is carried out at a temperature below the melting temperatures of the support and analysis materials, during which a pressing force in the direction normal to the lower and upper surfaces of the matrix is exerted on at least one portion of the matrix which adjoins said at least one pad inserted into the matrix and/or on at least one of the lower and upper surfaces of the at least one pad inserted into the matrix, said mechanical assembly of the at least one pad with the matrix resulting in the at least one pad being crimped on at least a portion of its lower and upper surfaces by the matrix.