Obtaining highly concentrated hmo solutions by reverse osmosis

A method for concentrating aqueous solutions of human milk oligosaccharides comprising subjecting an aqueous solution comprising at least one human milk oligosaccharide to a reverse osmosis process by means of a reverse osmosis membrane and concentrating the at least one human milk oligosaccharide to a final osmotic pressure of at least 70 bar measured with a freezing point osmometer.

A method comprising utilizing a reverse osmosis membrane in a reverse osmosis process for concentrating aqueous solutions of human milk oligosaccharides to final osmotic pressures of at least 70 bar measured with a freezing point osmometer.

A reverse osmosis system containing

• a RO membrane, especially a dairy RO membrane, and
• an aqueous solution comprising at least one HMO, especially 2′-FL, and having an osmotic pressure of at least 70 bar measured with a freezing point osmometer.