Non-human animals having a humanized clec9a gene
OwnerSokol Haxhinasto, Roberto Donnianniinventors
Life of this patent
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Abstract
Disclosed herein is a genetically modified rodent animal comprising a humanized Clec9a gene in its genome. Also disclosed herein are an isolated rodent tissue or cell including a rodent embryonic stem cell, a rodent embryo, a method of making the genetically modified rodent animal, and methods of using the genetically modified rodent animal.
Claims37
A genetically modified rodent animal comprising in its genome:
- a humanized Clec9a gene comprising:
- a rodent Clec9a nucleic acid sequence, and
- a human CLEC9A nucleic acid sequence,
- wherein the humanized Clec9a gene encodes a humanized Clec9a polypeptide comprising an ectodomain at least 90% identical in sequence to the ectodomain of a human CLEC9A protein, and
- wherein the rodent animal is a mouse or a rat.
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An isolated rodent tissue or cell, whose genome comprises a humanized Clec9a gene comprising a rodent Clec9a nucleic acid sequence and a human CLEC9A nucleic acid sequence, wherein the humanized Clec9a gene encodes a humanized Clec9a polypeptide comprising an ectodomain at least 90% identical in sequence to the ectodomain of a human CLEC9A protein, and wherein the rodent tissue or cell is a mouse tissue or cell, or a rat tissue or cell.
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A method of making a genetically modified rodent animal, comprising:
- modifying a rodent genome to comprise a humanized Clec9a gene, wherein the humanized Clec9a gene comprises a rodent Clec9a nucleic acid sequence and a human CLEC9A nucleic acid sequence, and encodes a humanized Clec9a polypeptide comprising an ectodomain at least 90% identical in sequence with the ectodomain of a human CLEC9A protein, and wherein the rodent animal is a mouse or a rat;
- and
- making the rodent animal comprising the modified rodent genome.
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A targeting nucleic acid construct, comprising
- a human CLEC9A nucleic acid sequence to be integrated into a rodent Clec9a gene at an endogenous rodent Clec9a locus, flanked by a 5′ nucleotide sequence and a 3′ nucleotide sequence that are homologous to nucleotide sequences at the rodent Clec9a locus,
- wherein integration of the human CLEC9A nucleic acid sequence into the rodent Clec9a gene results in a replacement of a rodent Clec9a genomic DNA with the human CLEC9A nucleic acid sequence thereby forming a humanized Clec9a gene, and
- wherein the humanized Clec9a gene encodes a humanized Clec9a polypeptide comprising an ectodomain substantially identical with the ectodomain of a human CLEC9A protein.
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Dependencies are inferred from the claim text.