Engineered bacterial cells and methods of producing the same

A method of engineering a target-biomolecule-producing bacterial cell, the method comprising:

• (i) identifying a set of optimized parameters predicted to result in increased production of the target biomolecule by the bacterial cell;
• (ii) constructing a plurality of bacterial strains, each bacterial strain comprising one or more of the optimized parameters of the set of optimized parameters identified in (i);
• (iii) collecting target biomolecule production data from the strains constructed in (ii);
• (iv) performing a computational analysis of the data collected in (iii) in order to obtain a further optimized set of parameters that predict increased production of the target biomolecule.
• (v) repeating steps (ii), (iii), and (iv);
• and
• (vi) constructing one or more final bacterial strains, each bacterial strain comprising one or more of the optimized parameters of the set of optimized parameters identified in (i) or (iv).