Method for analysing biological samples

A non-transitory computer-readable medium having program steps stored thereon, the program steps, when executed by a computer processor, causing performance of a method for analysing a biological sample with a plurality of analytes, the method comprising:

• determining information about the biological sample and the analytes, the analytes being marked by a plurality of markers,
• generating a probabilistic model of a distribution of the analytes within the biological sample based on the determined information,
• generating at least one optical readout of the biological sample, and
• determining a presence of at least one analyte in the at least one optical readout based at least partially on the probabilistic model of the distribution of the analytes within the biological sample.