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US 2023/0408525 A1Published application
Multifunctional magneto-polymeric nanosystems for rapid targeting, isolation, detection and simultaneous imaging of circulating tumor cells
OwnerJayant Jagannath KHANDARE, Shashwat BANERJEE +2inventors
InventorsJayant Jagannath KHANDARE, Shashwat BANERJEE, Muralidhara PADIGARU, Ganesh KHUTALE
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Abstract
A biofunctional multicomponent nanosystem for specific targeting, rapid isolation and simultaneous high resolution imaging of cancer cells is disclosed.
Claims9
A method of diagnosing cancer comprising:
- providing a biofunctional multicomponent nanosystem and a biological sample from a subject to be tested;
- detecting for the binding of the biofunctional multicomponent nanosystem to a cancer antigen, wherein an increase in detection of the cancer antigen relative to a control is indicative of cancer,
- wherein the biofunctional multicomponent nanosystem comprises a single or multi-walled carbon nanotube conjugated to a poly (amidoamine) (PAMAM) fourth generation (G4) dendrimer, which is covalently coupled to:
- (i) a glutathione (GSH) linker, including an iron oxide (Fe3O4) nanoparticle, which is further coupled to a circulating tumor cell (CTC)-specific anti-epithelial cell adhesion molecule (EpCAM) antibody;
- (ii) a poly(N-isopropylacrylamide) (PNIPAM);
- and
- (iii) a cyanine 5 NHS (Cy5) fluorescent dye moiety,
- wherein (i), (ii), and (iii), respectively, are coupled directly to the PAMAM G4 dendrimer.
5 more elements
A method of diagnosing myocardial infarction comprising:
- providing a biofunctional multicomponent nanosystem and a blood sample from a subject to be tested;
- detecting for the binding of the biofunctional multicomponent nanosystem to Troponin T in the blood sample, wherein an increase in detected Troponin T levels relative to a control is indicative of myocardial infarction in the subject,
- wherein the biofunctional multicomponent nanosystem comprises a single or multi-walled carbon nanotube conjugated to a poly (amidoamine) (PAMAM) fourth generation (G4) dendrimer, which is covalently coupled to:
- (i) a glutathione (GSH) linker, including an iron oxide (Fe3O4) nanoparticle, which is coupled to a circulating tumor cell (CTC)-specific anti-epithelial cell adhesion molecule (EpCAM) antibody;
- (ii) a poly(N-isopropylacrylamide) (PNIPAM);
- and
- (iii) a cyanine 5 NHS (Cy5) fluorescent dye moiety,
- wherein (i), (ii), and (iii), respectively, are coupled directly to the PAMAM G4 dendrimer.
5 more elements
A method of diagnosis an infectious disease in a subject comprising:
- providing a biofunctional multicomponent nanosystem and a biological sample from a subject to be tested;
- detecting for the binding of the biofunctional multicomponent nanosystem to a pathogenic organism relative to a control is indicative of an infectious disease in the subject,
- wherein the biofunctional multicomponent nanosystem comprises a single or multi-walled carbon nanotube conjugated to a poly (amidoamine) (PAMAM) fourth generation (G4) dendrimer, which is covalently coupled to:
- (i) a glutathione (GSH) linker, including an iron oxide (Fe3O4) nanoparticle, which is coupled to a circulating tumor cell (CTC)-specific anti-epithelial cell adhesion molecule (EpCAM) antibody;
- (ii) a poly(N-isopropylacrylamide) (PNIPAM);
- and
- (iii) a cyanine 5 NHS (Cy5) fluorescent dye moiety,
- wherein (i), (ii), and (iii), respectively, are coupled directly to the PAMAM G4 dendrimer.
5 more elements
Dependencies are inferred from the claim text.