US 2023/0408524 A1 · REAGENT COMPOSITION FOR DETECTION OF NON-HEMATOPOIETIC TUMOR AND USE THEREOF

Abstract

The present invention provides a reagent composition for detection of a non-hematopoietic tumor and use thereof. Said reagent composition includes three sets of antibodies, with the first set of antibodies including an anti-CD9 antibody, an anti-GD2 antibody, an anti-CD3 antibody, an anti-CD4 antibody, an anti-CD56 antibody, an anti-CD36 antibody, an anti-CD81, and an anti-CD45 antibody; the second set of antibodies including an anti-HLA-ABC antibody, an anti-CD38 antibody, an anti-CD19 antibody, an anti-CD56 antibody, an anti-CD36 antibody, an anti-CD7 antibody, and an anti-CD45 antibody; the third set of antibodies including anti-cytoplasmic cytokeratin antibodies; wherein the first set of antibodies and the second set of antibodies are respectively used for samples in separate tubes, and the third set of antibodies is used for the sample in the same tube as the second set of antibodies. The reagent composition of the present invention can be applied for flow cytometry screening, diagnosis and/or follow-up detection of a non-hematopoietic tumor.

Claims16

Dependent claims

A reagent composition that can be used for detection of a non-hematopoietic tumor by flow cytometry, wherein the reagent composition includes a first set of antibodies, a second set of antibodies, and a third set of antibodies, wherein:

the first set of antibodies includes:
an anti-CD9 antibody, an anti-GD2 antibody, an anti-CD3 antibody, an anti-CD4 antibody, an anti-CD56 antibody, an anti-CD36 antibody, an anti-CD81 antibody, and an anti-CD45 antibody;
and the first set of antibodies is to be added to a first flow cytometric tube in which a sample to be assayed is in the form of a single cell suspension;
the second set of antibodies includes:
an anti-HLA-ABC antibody, an anti-CD38 antibody, an anti-CD19 antibody, an anti-CD56 antibody, an anti-CD36 antibody, an anti-CD7 antibody, and an anti-CD45 antibody;
and the second set of antibodies is to be added to a second flow cytometric tube in which a sample to be assayed is in the form of a single cell suspension;
and
the third set of antibodies includes:
anti-cytoplasmic cytokeratin antibodies;
and the third set of antibodies is to be added to the second flow cytometric tube to which the second set of antibodies has been added and permeabilization has been performed.

7 more elements

A method for detecting a non-hematopoietic tumor by flow cytometry, comprising preparing a flow cytometry loading sample after processing a sample to be tested using a reagent composition,

wherein the reagent composition includes a first set of antibodies, a second set of antibodies, and a third set of antibodies, wherein,
the first set of antibodies includes:
an anti-CD9 antibody, an anti-GD2 antibody, an anti-CD3 antibody, an anti-CD4 antibody, an anti-CD56 antibody, an anti-CD36 antibody, an anti-CD81 antibody, and an anti-CD45 antibody;
and the first set of antibodies is to be added to a first flow cytometric tube in which a sample to be assayed is in the form of a single cell suspension,
the second set of antibodies includes:
an anti-HLA-ABC antibody, an anti-CD38 antibody, an anti-CD19 antibody, an anti-CD56 antibody, an anti-CD36 antibody, an anti-CD7 antibody, and an anti-CD45 antibody;
and the second set of antibodies is to be added to a second flow cytometric tube in which a sample to be assayed is in the form of a single cell suspension,
the third set of antibodies includes:
anti-cytoplasmic cytokeratin antibodies;
and the third set of antibodies is to be added to the second flow cytometric tube to which the second set of antibodies has been added and permeabilization has been performed.

7 more elements

Dependencies are inferred from the claim text.

Reagent composition for detection of non-hematopoietic tumor and use thereof

Life of this patent

Reagent composition for detection of non-hematopoietic tumor and use thereof

Life of this patent

Abstract

Claims16