Developing lateral flow immunochromatography (lfia) peptide-based test strips for rapid detection of antigens and antibodies against specific antigens

A method of fabricating a lateral flow immunoassay (LFIA) comprising:

• synthesizing and screening at least one peptide antigen;
• conjugating the peptide antigen with at least one tag;
• printing a test strip;
• determining the minimum thickness of a test cassette;
• and
• assembling the LFIA,
• wherein the peptide antigen binds specifically to at least one analyst,
• wherein at least one control line and at least one test line are printed on the test strip, and
• wherein the material in the test line binds the complex formed by the analyst and the peptide antigen conjugated with the tag, wherein the epitopes are at least one selected from the group consisting of peptides with amino acid sequences provided in SEQ ID Nos. 1-10 and 10-19.

A lateral flow immunoassay (LFIA) comprising:

• at least one fusion-peptide comprising at least two epitopes of SARS-CoV-2, wherein the epitopes are linked by peptide bond, wherein the epitopes are at least one selected from the group consisting of peptides with amino acid sequences provided in SEQ ID Nos. 1-10 and 10-19;
• a test strip;
• a tag;
• and
• a cassette,
• wherein the fusion-peptide binds specifically to at least one analyst,
• wherein the fusion-peptide is conjugated with the tag,
• wherein the test strip has at least one control line and at least one test line, and
• wherein the material in the test line can bind the complex formed by the analyst and the peptide antigen conjugated with the tag.