Compositions and methods involving antibody constructs
AssigneesCZ Biohub SF, LLC, The Board of Trustees of the Leland Stanford Junior University
InventorsJing Pan, Ian Andrew Paul Thompson, Brandon Wilson, Hyongsok Tom Soh, Nicolo Maganzini
Life of this patent
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Abstract
The disclosure provides antibody constructs that can reversibly bind to biological or chemical target analytes. Upon target analyte binding, the antibody construct can change its conformational state to produce a detectable readout. An antibody construct can be a single-antibody construct or a dual-antibody construct.
Claims34
An antibody construct comprising:
- an antibody or a binding fragment thereof comprising a first label, wherein the antibody or the binding fragment thereof is linked to a blocking analyte and a second label via a linker,
- wherein:
- in the absence of a target analyte, the blocking analyte binds to the antibody or the binding fragment thereof, and
- in the presence of the target analyte, the target analyte competes with the blocking analyte for binding to the antibody or the binding fragment thereof, and
- the first and second labels interact to generate a detectable readout that changes depending on whether the blocking analyte is bound by the antibody or the binding fragment thereof, or is not bound by the antibody or the binding fragment thereof.
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An antibody construct comprising:
- an antibody or a binding fragment thereof comprising a redox reporter, wherein the antibody or the binding fragment thereof is linked to a blocking analyte and a sensing electrode;
- wherein:
- in the absence of a target analyte, the blocking analyte binds to the antibody or the binding fragment thereof, and
- in the presence of the target analyte, the target analyte competes with the blocking analyte for binding to the antibody or the binding fragment thereof, and
- the redox reporter and the sensing electrode interact to generate an electrical signal that changes depending on whether the blocking analyte is bound by the antibody or the binding fragment thereof, or is not bound by the antibody or the binding fragment thereof.
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An antibody construct comprising:
- (a) two detecting strands, wherein a first detecting strand comprises a first antibody or a binding fragment thereof, and a second detecting strand comprises a second antibody or a binding fragment thereof;
- and
- (b) a first label and a second label,
- wherein the detecting strands or portions thereof are complementary and hybridize to each other or one or more scaffold strands, and
- wherein in the presence of the target analyte, the first antibody and the second antibody bind to two different epitopes on the target analyte, and the first and second labels interact with each other to generate a detectable readout compared to when there is an absence of the target analyte.
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Dependencies are inferred from the claim text.