Method of analyzing neurons, device for analyzing neurons, and computer program

A method of analyzing neurons, comprising:

• acquiring an image by acquiring time-series images obtained by imaging a neuron in time series;
• identifying a cell region by identifying a region of a neurite or a region of a cell body of the neuron on a basis of the time-series images;
• detecting aggregates by detecting, on a basis of the time-series images obtained by imaging, in time series, first fluorescent protein tagged to specific protein expressed in the neuron, presence or absence of aggregates of the specific protein aggregated in the region of the neurite or the region of the cell body identified in the identifying the cell region by using, as a basis, luminance of the first fluorescent protein included in the time-series images;
• and
• performing an analysis by classifying the neuron into a plurality of groups on a basis of a detection result of the detecting the aggregates and analyzing a survival state of the neuron for each of the groups, wherein
• the survival state of the neuron includes at least any one of a survival period of time or a survival rate of the neuron, and
• the performing the analysis includes displaying, for each of the groups, a relationship between the plurality of groups and survival states of the plurality of groups.